In addition to generating antibody libraries from naive B cell banks, germline sequences or immune donors, the diversity of antibody libraries can be improved by using available information about antibody structures to include short random sequences in carefully selected antibody slices. 

Overview of Semi-synthetic Antibody Library

Semi-synthetic antibody library is an antibody library composed of a part of synthetic variable region sequences and another part of natural sequences. The main purpose of constructing a semi synthetic antibody library is to optimize the sequence of antibodies. The construction of such antibody libraries is mainly to use the heavy chain, light chain or rearranged variable region fragments of the germline, and one or more CDRs are randomly rearranged. The CDR region of the antibody can be partially or completely randomized by direct oligonucleotide mutation or PCR based methods, including CDR3 sequence randomization, CDR3 length randomization, and all CDR regions randomization.

Using semi synthetic antibody library to prepare antibodies without immunization has good application prospects for the development of human monoclonal antibodies, especially for various receptor factors, receptors and other autoantigens that are difficult to immunize in vivo. Affinity is an important factor in preparing antibodies with this technology. Generally speaking, the larger the reservoir, the more likely to obtain high affinity antibodies.\

Learn more：semi-synthetic antibody library construction services